Three-Dimensional linear and nonlinear transformations: An integration of light microscopical and MRI data

摘要: The registration of image volumes derived from different imaging modalities such as MRI, PET, SPECT, and CT has been described in numerous studies which functional morphological data are combined on the basis macrostructural information. However, exact topography architectural details is defined by microstructural information histological sections. Therefore, a technique developed for integrating micro- based 1) three-dimensional reconstruction volume accounts linear nonlinear deformations, 2) two-step procedure transforms these to reference coordinate system. uses an extended principal axes transformation (PAT) generalized affine transformations fast, automated full-multigrid method (FMG) determining high-dimensional deformations order account differences morphology individuals. With this technique, it possible define upwards 1,000 times resolution approximately 1 mm making identification geometric texture features microscopically brain structures.