A gamma-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex.
作者： E Sigel , F A Stephenson , C Mamalaki , E A Barnard
DOI: 10.1016/S0021-9258(18)32319-6
关键词:
摘要: The gamma-aminobutyric acid/benzodiazepine receptor from bovine cerebral cortex was solubilized with sodium deoxycholate and purified by affinity chromatography on benzodiazepine-agarose ion exchange chromatography. benzodiazepine binding protein enriched 1800-fold. Polyacrylamide gel electrophoresis in the presence of dodecyl sulfate dithiothreitol showed two major bands Mr = 57,000 53,000. [3H]Flunitrazepam, after UV irradiation, incorporated irreversibly into both isolated protein. A high site for acid co-purified sites were shown to reside same physical structure. dissociation constants 10 +/- 4 nM [3H] flunitrazepam 12 3 agonist [3H]muscimol. maximum specific activity muscimol 4.3 nmol/mg ratio [3H]muscimol [3H]flunitrazepam between 4. Gel filtration sucrose density gradient sedimentation studies gave a Stokes radius 7.3 0.5 nm coefficient 11.1 0.3 S, respectively. complex had pharmacological profile that corresponds specificity found membranes crude soluble extracts.
sci-hub.st 参考文章(33)
J.W. Thomas, J.F. Tallman, Characterization of photoaffinity labeling of benzodiazepine binding sites. Journal of Biological Chemistry. ,vol. 256, pp. 9838- 9842 ,(1981) , 10.1016/S0021-9258(19)68705-3
S. C. Lummis, C. Napias, D. V. Greenlee, M. O. Bergman, P. C. van Ness, A. E. Watkins, R. W. Olsen, γ-Aminobutyric Acid Receptor Binding in Mammalian Brain: Heterogeneity of Binding Sites Molecular Pharmacology. ,vol. 19, pp. 217- 227 ,(1981)
RICHARD F. SQUIRES, CLAUS BRAESTRUP, Benzodiazepine receptors in rat brain Nature. ,vol. 266, pp. 732- 734 ,(1977) , 10.1038/266732A0
F. Anne STEPHENSON, Andrew E. WATKINS, Richard W. OLSEN, Physicochemical characterization of detergent-solubilized gamma-aminobutyric acid and benzodiazepine receptor proteins from bovine brain. FEBS Journal. ,vol. 123, pp. 291- 298 ,(1982) , 10.1111/J.1432-1033.1982.TB19766.X
H. Mohler, M. K. Battersby, J. G. Richards, Benzodiazepine receptor protein identified and visualized in brain tissue by a photoaffinity label. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 1666- 1670 ,(1980) , 10.1073/PNAS.77.3.1666
Li-Ren Chang, Eric A. Barnard, Mathew M.S. Lo, J.Oliver Dolly, Molecular sizes of benzodiazepine receptors and the interacting gaba receptors in the membrane are identical FEBS Letters. ,vol. 126, pp. 309- 312 ,(1981) , 10.1016/0014-5793(81)80268-2
M. G. Douglas, R. A. Butow, Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 1083- 1086 ,(1976) , 10.1073/PNAS.73.4.1083
C. BRAESTRUP, R. ALBRECHTSEN, R. F. SQUIRES, High densities of benzodiazepine receptors in human cortical areas Nature. ,vol. 269, pp. 702- 704 ,(1977) , 10.1038/269702A0
Donald V. Greenlee, Paul C. Ness, Richard W. Olsen, Gamma-aminobutyric acid binding in mammalian brain: receptor-like specificity of sodium-independent sites. Journal of Neurochemistry. ,vol. 31, pp. 933- 938 ,(1978) , 10.1111/J.1471-4159.1978.TB00130.X
Bethan Lang, Eric A. Barnard, Li-Ren Chang, J.Oliver Dolly, Putative benzodiazepine receptor: A protein solubilised from brain FEBS Letters. ,vol. 104, pp. 149- 153 ,(1979) , 10.1016/0014-5793(79)81103-5