Flow cytometry-based quantification of cytokine levels in AML patients plasma and cell culture supernatants.

摘要: AIM Bone marrow stromal cells (BMSCs) have been found to support leukemic cell survival; however, the mechanisms responsible are far from being elucidated yet. The main aim of current study is identify particular cytokine/chemokine patterns acute myeloid leukemia (AML) cells, and, on a longer term, correlate them with patient outcome and response therapy. Therefore, influence BMSCs in vitro modulation cytokine secretion (IL-1beta, TNF-alpha, IL-10, IFN-gamma, IL-4, IL-5, IL-2) by AML as well supportive capacity BMSCs-derived soluble factors was investigated. MATERIAL AND METHODS With this purpose, we used an experimental model consisting evaluation effect BMSC confluent layers-conditioning medium (BMSC-CM) M4/5 cultures. RESULTS Our results show that BMSC-CM both patients healthy subjects conferred substantial beneficial throughout culture (p=0.0002 0.0020 respectively at 24 hours p=0,0013 0,0030 72 hours), temporary increase viability BM plasma patients. Significant differences were observed respect IL1 b which upregulated cultures after following addition AML-BMSC-CM, contrast control-BMSC-CM. CONCLUSION suggest contribution generation may be key players involved vivo maintenance malignant clone.