The Effect of Chemoradiotherapy with SRC Tyrosine Kinase Inhibitor, PP2 and Temozolomide on Malignant Glioma Cells In Vitro and In Vivo

摘要: Purpose We investigated the effect of chemoradiotherapy with PP2 and temozolomide (TMZ) on malignant glioma cells using clonogenic assays in vivo brain tumor model. Materials methods The radiosensitivity U251 T98G was assays. expression E-cadherin, matrix metalloproteinases 2 (MMP2), Ephrin type-A receptor (EphA2), vascular endothelial growth factor (VEGF) measured by Western blotting an accumulation γH2AX foci 6 hours after radiotherapy treatment. migration, invasion, vasculogenic mimicry formation (VMF) evaluated. In orthotopical model cells, injected intraperitoneally or without oral TMZ before, during whole radiotherapy. Bioluminescence images were taken to visualize tumors immunohistochemical staining VEGF, CD31, EphA2, hypoxia-inducible 1a performed. Results increased decreasing survival normal human astrocytes. Chemoradiotherapy resulted foci. induced overexpression E-cadherin suppression MMP2, EphA2. also compromised VMF cells. tumors, decreased volume best, but not statistically significantly compared TMZ. VEGF CD31 suppressed PP2-treated tumors. Conclusion enhances suppresses invasion migration non-significant decrease.