Osmotic response of oocytes using a microscope diffusion chamber: a preliminary study comparing murine and human ova
作者： A. Bernard , J.J. McGrath , B.J. Fuller , D. Imoedemhe , R.W. Shaw
DOI: 10.1016/0011-2240(88)90295-7
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摘要: Abstract The hydraulic conductivity (Lp) of the plasma membrane determines how cells respond to stresses dehydration encountered during cryopreservation. We have used a microscope diffusion chamber which allows for direct real-time observation dynamic osmotic response individual in microvolume suspension compare Lp murine and human unfertilized ova. In this system, an cell induced imbalance is documented via series photomicrographs or videotape; from these data can be computed. Donated preovulatory oocytes were compared with macroscopically normal ova, 43 hr after insemination, had failed fertilize (Ff) ova collected 13 post chorionic gonadotropin injection. permeability coefficients 0.65 ± 0.43, 0.84 0.39, 0.36 0.07 (μm3/μm2/atm/min. results suggest that it may possible use Ff experiments design suitable cryopreservation procedures.
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