Conjugated linoleic acids have no effect on TNFα-induced adhesion molecule expression, U937 monocyte adhesion, and chemokine release in human aortic endothelial cells

摘要: Abstract Leukocyte recruitment and adhesion to the endothelium are critical steps in early phase of atherosclerosis. Synthetic ligands peroxisome proliferator-activated receptors (PPARs) were shown reduce cytokine-stimulated leukocyte–endothelial cell interactions by inhibiting NF-κB mediated inflammatory response. Conjugated linoleic acids (CLA), which natural PPARs, demonstrated have anti-inflammatory anti-atherogenic properties vivo. With a view elucidating mechanisms CLA, present study aimed explore effects cis -9, trans -11 CLA -10, -12 on cytokine-induced chemokine release, surface expression molecules (ICAM-1, VCAM-1, E-selectin) U937 monocyte human aortic endothelial cells (HAEC). Treatment HAECs with 2 ng/mL TNFα markedly increased molecules, adhesion, release chemoattractant protein (MCP)-1. However, treatment either isomer or acid did not modulate ICAM-1, E-selectin, MCP-1 release. In addition, both isomers slightly PPARγ DNA-binding activity, but alter activity NF-κB. conclusion, showed no effect monocyte–endothelial molecular regulating these processes HAEC. This suggests that observed vivo probably reduced interactions.