Zebrafish kit mutation reveals primary and secondary regulation of melanocyte development during fin stripe regeneration.

摘要: Fin regeneration in adult zebrafish is accompanied by re-establishment of the pigment stripes. To understand mechanisms underlying fin stripe and regulation normal melanocyte morphology, we investigated origins melanocytes regenerating their requirement for kit receptor tyrosine kinase. Using pre-existing melanin as a lineage tracer, show that most develop from undifferentiated precursors, rather than differentiated melanocytes. Mutational analysis reveals two distinct classes First, an early class develops dependent on function. In absence function kit-dependent melanocytes, second at later stages regeneration. This late kit-independent has little or no role wild-type development, thus revealing secondary mode Expression markers mutant fins suggests normally acts after mitf before dct to promote development primary are also present during ontogeny patterns similar those observed