Purification and some properties of sulfur:ferric ion oxidoreductase from Thiobacillus ferrooxidans.
作者： T Sugio , W Mizunashi , K Inagaki , T Tano
DOI: 10.1128/JB.169.11.4916-4922.1987
关键词:
摘要: Sulfite:ferric ion oxidoreductase in the plasma membrane of Thiobacillus ferrooxidans AP19-3 was purified to an electrophoretically homogeneous state. The enzyme had apparent molecular weight 650,000 and composed two subunits (M(rs), 61,000 59,000) as estimated by sodium sulfate-polyacrylamide gel electrophoresis. Michaelis constants sulfite:ferric for Fe3+ sulfite ions were 1.0 0.071 mM, respectively. suffered from end product inhibition 1 mM Fe2+.
core.ac.uk
elsevier.com
highwire.org参考文章(12)
W M Southerland, F Toghrol, Sulfite oxidase activity in Thiobacillus novellus. Journal of Bacteriology. ,vol. 156, pp. 941- 944 ,(1983) , 10.1128/JB.156.2.941-944.1983
F Toghrol, W M Southerland, Purification of Thiobacillus novellus sulfite oxidase. Evidence for the presence of heme and molybdenum. Journal of Biological Chemistry. ,vol. 258, pp. 6762- 6766 ,(1983) , 10.1016/S0021-9258(18)32286-5
Frank W. Adair, Membrane-associated Sulfur Oxidation by the Autotroph Thiobacillus thiooxidans Journal of Bacteriology. ,vol. 92, pp. 899- 904 ,(1966) , 10.1128/JB.92.4.899-904.1966
T Sugio, T Katagiri, M Moriyama, Y L Zhèn, K Inagaki, T Tano, Existence of a new type of sulfite oxidase which utilizes ferric ions as an electron acceptor in Thiobacillus ferrooxidans Applied and Environmental Microbiology. ,vol. 54, pp. 153- 157 ,(1988) , 10.1128/AEM.54.1.153-157.1988
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Ronald M. Lyric, Isamu Suzuki, Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. I. Survey of enzymes and properties of sulfite : cytochrome c oxidoreductase Canadian Journal of Biochemistry. ,vol. 48, pp. 334- 343 ,(1970) , 10.1139/O70-056
J. London, S. C. Rittenberg, PATH OF SULFUR IN SULFIDE AND THIOSULFATE OXIDATION BY THIOBACILLI Proceedings of the National Academy of Sciences of the United States of America. ,vol. 52, pp. 1183- 1190 ,(1964) , 10.1073/PNAS.52.5.1183
D.J.W. Moriarty, D.J.D. Nicholas, Electron transfer during sulphide and sulphite oxidation by Thiobacillus concretivorus Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 216, pp. 130- 138 ,(1970) , 10.1016/0005-2728(70)90165-9
J. Robie Vestal, D. G. Lundgren, The sulfite oxidase of Thiobacillus ferrooxidans (Ferrobacillus ferrooxidans) Biochemistry and Cell Biology. ,vol. 49, pp. 1125- 1130 ,(1971) , 10.1139/O71-162