Heat shock and ecdysterone activation of the Drosophila melanogaster hsp23 gene; a sequence element implied in developmental regulation.

摘要: The regulation of the Drosophila melanogaster hsp23 gene by heat shock and ecdysterone has been analysed measuring activities hsp--Escherichia coli beta-galactosidase hybrid genes in transfected hormone-sensitive D. cells. Mutation analysis identified multiple, distinct promoter elements. A sequence element, which also occurs promoters several other developmentally regulated genes, is present regions that are essential for its ecdysterone, but not heat-regulated activity; this element may represent a binding site an ecdysterone--receptor complex. Mutant can be activated only or hormone have constructed. Thus two types function independently each other.