Effects of mutations in Drosophila nicotinic acetylcholine receptor subunits on sensitivity to insecticides targeting nicotinic acetylcholine receptors

摘要: Abstract Several strains of Drosophila melanogaster possess mutant alleles in nicotinic acetylcholine receptor (nAChR) subunits, Dα1 and Dβ2 that confer resistance to neonicotinoids such as imidacloprid nitenpyram, Dα6, confers spinosyns. These were bioassayed with a selected set nAChR active insecticides including neonicotinoids, spinosad, sulfoxaflor, new sulfoximine insecticide. All the examined, except dinotefuran showed reduced insecticidal efficacy on larvae mutant, suggesting this subunit may be important action these insecticides. dinotefuran, possessing mutation. A similar pattern broad neonicotinoid alone was also observed for both mutations (Dα1 + Dβ2). The mutation exhibited lower level cross-resistance sulfoxaflor ( 13-fold). In contrast, there no any or adult flies Dα6 mutation, which high levels spinosad. Thus D. studied, target site spinosyns does not translate directly towards sulfoxaflor.