Fat Mass and Obesity-Associated (FTO) Stimulates Osteogenic Differentiation of C3H10T1/2 Cells by Inducing Mild Endoplasmic Reticulum Stress via a Positive Feedback Loop with p-AMPK.

摘要: Fat mass and obesity-associated (FTO) gene helps to regulate energy homeostasis in mammals by controlling expenditure. In addition, FTO functions the regulation of obesity adipogenic differentiation; however, a role osteogenic differentiation is unknown. This study investigated effects on C3H10T1/2 cells underlying mechanism. Expression endoplasmic reticulum (ER) stress markers were characterized reverse-transcriptase polymerase chain reaction western blotting. Alkaline phosphatase (ALP) staining was performed assess ALP activity. BMP2 treatment increased mRNA expression genes FTO. Overexpression distal-less homeobox5 (Dlx5) runt-related transcription factor 2 (Runx2). Activation adenosine monophosphate-activated protein kinase (AMPK) expression, there positive feedback loop between p-AMPK. p-AMPK induced mild ER stress; tunicamycin-induced severe suppressed AMPK activation. summary, induces upon inducing via with activation induce stress. By contrast, inhibits suppressing