Discerning Different In vivo Roles of MicroRNAs by Experimental Approaches in Zebrafish

摘要: MicroRNAs (miRNAs) are small endogenous RNAs of approximately 23 nucleotides that regulate the cellular transcriptome by binding to target mRNAs in a sequence-restricted manner, thereby modulating transcript translation and turnover. Although direct repressive trans-acting action miRNAs is cause net reduction total amount protein generated from their mRNAs, developmental physiological processes have combined this with flexibility spatial temporal regulation both targets employ range regulatory roles. These different roles achieve diverse outcomes. Five common vivo summarized, along key defining attributes could be experimentally addressed distinguish between them. Methods utilizing zebrafish suitable for determining functional role particular miRNA interest outlined.