Polyhydroxyalkanoate (PHA) production using waste vegetable oil by Pseudomonas sp. strain DR2.

摘要: To produce polyhydroxyalkanoate (PHA) from inexpensive substrates by bacteria, vegetable-oil-degrading bacteria were isolated a rice field using enrichment cultivation. The Pseudomonas sp. strain DR2 showed clear orange or red spots of accumulated PHA granules when grown on phosphate and nitrogen limited medium containing vegetable oil as the sole carbon source stained with Nile blue A. Up to 37.34% (w/w) intracellular was produced corn oil, which consisted three major 3-hydroxyalkanoates; octanoic (C8:0, 37.75% total 3-hydroxyalkanoate content PHA), decanoic (C10:0, 36.74%), dodecanoic (C12:0, 11.36%). up 23.52% PHAMCL waste oil. proportion 3- hydroxyalkanoate vegetable-oil-derived [hexanoic (5.86%), (45.67%), (34.88%), tetradecanoic (8.35%), hexadecanoic (5.24%)] composition ratio different that corn-oil-derived PHA. Strain used fatty acids in same ratio, linoleic acid production. Interestingly, production could not occur either acetate- butyrate-amended media. greater amount than other well-studied strains (Chromobacterium violaceum Ralstonia eutropha H16) data capable producing