Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco

摘要: cDNAs encoding three proteins from barley (Hordeum vulgare), a class-II chitinase (CHI), beta-1,3-glucanase (GLU) and Type-I ribosome-inactivating protein (RIP) were expressed in tobacco plants under the control of CaMV 35S-promoter. High-level expression transferred genes was detected transgenic by Northern Western blot analysis. The leader peptides CHI GLU led to accumulation these intercellular space leaves. RIP, which is naturally deposited cytosol endosperm cells, either its original cytosolic form or fused plant secretion peptide (spRIP). Fungal infection assays revealed that individual each case resulted an increased protection against soilborne fungal pathogen Rhizoctonia solani, infects range species including tobacco. To create situation similar 'multi-gene' tolerance, traditional breeding experience has shown provide crops with longer-lasting protection, several antifungal combined attack resulting their co-expression planta evaluated. Transgenic lines generated tandemly arranged coding for RIP as well CHI. performance co-expressing transgenes GLU/CHI CHI/RIP solani assay significantly enhanced when compared levels obtained corresponding isogenic expressing single transgene level. data indicate synergistic protective interaction co-expressed vivo.