Protein Phosphatase-Dependent Circadian Regulation of Intermediate-Term Associative Memory

摘要: The endogenous circadian clock is a principal factor modulating memory across species. Determining the processes through which modulates formation key issue in understanding and identifying mechanisms to improve memory. We used marine mollusk Aplysia californica investigate modulation of intermediate-term (ITM) phase specifically suppresses using operant learning paradigm, that food inedible. found ITM, temporally mechanistically distinct form memory, rhythmically expressed under light-dark constant conditions when induced by either massed or spaced training. Strong regulation ITM occurs with exhibited only animals trained during early subjective day; no apparent training late day night. Given necessity multiple persistent kinase cascades for we investigated whether protein phosphatase activity affected modulation. Inhibition phosphatases 1 2A blocked were (subjective) while resulting phase-specific rescue In contrast, inhibition calcineurin did not block permitted day, evening, throughout These results demonstrate levels are critical regulators one mechanism regulates formation.