ISOLATION OF BACILLUS SUBTILIS MH-4 FROM SOIL AND ITS POTENTIAL OF POLYPEPTIDIC ANTIBIOTIC PRODUCTION
作者： Bushra Jamil , Fariha Hasan , A Hameed , Safia Ahmed
DOI:
关键词:
摘要: The genus Bacillus produces mainly polypeptide antibiotics such as bacitracin and polymyxin. species were isolated from soil by sprinkle technique. And all screened for the production of antibiotic. subtilis MH-4 gave maximum antimicrobial activity so finally selected optimization. During optimization culture conditions best antibacterial was obtained at 96 hours incubation period, pH-8 using glycerol carbon L-glutamic acid nitrogen source. Optimum temperature antibiotic 37 degrees C. confirmed to be paper chromatography. Antibiotic further extracted successfully with 1-Butanol, aqueous concentrate showed 0.8 mg/ml. produced found narrow spectrum active against only Gram-positive bacteria.
researchgate.net 参考文章(27)
Neva Snell, Kosuke Ijichi, J. C. Lewis, Paper chromatographic identification of polypeptidic gram positive inhibiting antibiotics. Applied and Environmental Microbiology. ,vol. 4, pp. 13- 17 ,(1956) , 10.1128/AM.4.1.13-17.1956
John E. Snoke, FORMATION OF BACITRACIN BY PROTOPLASTS OF BACILLUS LICHENIFORMIS. Journal of Bacteriology. ,vol. 81, pp. 986- 989 ,(1961) , 10.1128/JB.81.6.986-989.1961
Karanjit S. Kooner, Mordechai Sharir, Thom J. Zimmerman, Textbook of ocular pharmacology Lippincott-Raven. ,(1997)
Herbert S. Anker, Balbina A. Johnson, Joseph Goldberg, Frank L. Meleney, Bacitracin: Methods of Production, Concentration, and Partial Purification, with a Summary of the Chemical Properties of Crude Bacitracin Journal of Bacteriology. ,vol. 55, pp. 249- 255 ,(1948) , 10.1128/JB.55.2.249-255.1948
Andrea M. Neumüller, Dirk Konz, Mohamed A. Marahiel, The two-component regulatory system BacRS is associated with bacitracin 'self-resistance' of Bacillus licheniformis ATCC 10716. FEBS Journal. ,vol. 268, pp. 3180- 3189 ,(2001) , 10.1046/J.1432-1327.2001.02203.X
G W Hanlon, N A Hodges, Bacitracin and protease production in relation to sporulation during exponential growth of Bacillus licheniformis on poorly utilized carbon and nitrogen sources. Journal of Bacteriology. ,vol. 147, pp. 427- 431 ,(1981) , 10.1128/JB.147.2.427-431.1981
Torsten Stein, Bacillus subtilis antibiotics: structures, syntheses and specific functions Molecular Microbiology. ,vol. 56, pp. 845- 857 ,(2005) , 10.1111/J.1365-2958.2005.04587.X
Li-June Ming, Jon D. Epperson, Metal binding and structure-activity relationship of the metalloantibiotic peptide bacitracin. Journal of Inorganic Biochemistry. ,vol. 91, pp. 46- 58 ,(2002) , 10.1016/S0162-0134(02)00464-6
H. I. HAAVIK, Studies on the formation of bacitracin by Bacillus licheniformis: role of catabolite repression and organic acids. Microbiology. ,vol. 84, pp. 321- 326 ,(1974) , 10.1099/00221287-84-2-321
Graham Bell, Pierre-Henri Gouyon, Arming the enemy: the evolution of resistance to self-proteins Microbiology. ,vol. 149, pp. 1367- 1375 ,(2003) , 10.1099/MIC.0.26265-0