Starting a DNA barcode reference library for shallow water polychaetes from the southern European Atlantic coast.

摘要: Annelid polychaetes have been seldom the focus of dedicated DNA barcoding studies, despite their ecological relevance and often dominance, particularly in soft-bottom estuarine coastal marine ecosystems. Here, we report first assessment performance barcodes discrimination shallow water polychaete species from southern European Atlantic coast, focusing on specimens collected estuaries ecosystems Portugal. We analysed cytochrome oxidase I (COI-5P) 164 specimens, which were assigned to 51 morphospecies. To our data set Portugal, added available published sequences selected same species, genus or family, inspect for taxonomic congruence among studies collection location. The final comprised 290 79 morphospecies, generated 99 Barcode Index Numbers (BINs) within Life Data Systems (BOLD). Among these, 22 BINs singletons, 47 other concordant, confirming initial identification based morphological characters, 30 discordant, most consisted multiple found Some prominent cases latter category include Hediste diversicolor (O.F. Muller, 1776) (7), Eulalia viridis (Linnaeus, 1767) (2) Owenia fusiformis (delle Chiaje, 1844) (5), all them reported Portugal frequently used as environmental quality indicators. Our results these showed discordance between molecular lineages additional relatively divergent lineages. potential inaccuracies assessments, where underpinning diversity is poorly resolved clarified, demand extensive investigation barcode this group, parallel with alpha taxonomy efforts.