Synthesis of ROS scavenging microspheres from a dopamine containing poly(β-amino ester) for applications for neurodegenerative disorders.

摘要: Parkinson's disease (PD) is a common neurodegenerative characterized by substantial decrease of dopaminergic neurons in the substantia nigra pars compacta. The neurological deterioration during PD can be, part, attributed to elevated levels reactive oxygen species (ROS). Radical scavengers have previously been shown protect cells from toxic effects vitro. Hence, new approaches need be investigated improve administration antioxidants order provide neuroprotection. Polymers exhibiting catechol structures offer one such approach due their interesting physicochemical properties. In present study photocrosslinkable dopamine-containing poly(β-amino ester) (DPAE) was synthesized poly(ethylene glycol) diacrylate (PEGDA) and dopamine hydrochloride using Michael type addition. A water-in-oil emulsion technique used photo-crosslink polymer into spherical microparticles. DPAE microspheres featured excellent scavenging properties towards 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radicals dose dependent manner could even reduce dissolved content physiological solution. Furthermore, concentrations required for radical were non-toxic SH-SY5Y as well primary astrocytes embryonic rat ventral midbrain cultures.