Isolation, Functional Properties and Clonal Analysis of Tumor-Infiltrating Lymphocytes from Human Brain Tumors

摘要: Despite the numerous reports of decreased or defective systemic cell mediated immunity in glioma patients (1–7) there is evidence a local immune response to tumor. Thus mononuclear (MNC) infiltrates have been demonstrated within parenchyma human glial tumors 30–60 % cases reported literature (8, 9). Other studies correlated intensity MNC with tumor histology and survival (10–12). Von Hanwehr co-workers (9) found that T lymphocytes constituted major fraction gliomas. However nature functional capabilities these cells their potential cytolytic role preventing growth remain poorly understood. To resolve questions it necessary isolate infiltrating (TIL) from tissue. this end TIL 7 brain were isolated using enzyme digestion density gradient centrifugation. The resulting fractions then cloned limiting dilution assay (13) allows virtualy all peripheral blood resting undergo clonal expansion. frequency proliferating lymphocyte precursors (PTL-P) can be calculated thus giving an indication immunocompetent present original infiltrate compared patient normal blood. In addition clones obtained expanded allow for assays vitro. This report demonstrates gliomas grown presence interleukin-2 do exhibit activity vitro both allogeneic autologous systems.