Characterisation of degranulation and phagocytic capacity of a human neutrophilic cellular model, PLB-985 cells☆

摘要: Exocytosis of neutrophil granules is a major event that converts circulating neutrophils into fully activated cells capable chemotaxis, phagocytosis and destruction pathogens. The PLB-985 cell line suitable neutrophilic cellular model which utilised to study the different functional responses neutrophils. In this study, we characterised differentiation toward granulocytic pathway, using three inducing agents: dbcAMP, DMSO DMF. efficiency was monitored by observation morphology with electron microscopy, analysis expression receptors such as FPRL1 FcγRIIA, distribution or release granule markers, phagocytic capacity, well measurement fMLF-induced calcium fluxes. in differentiated were weaker compared fMLF stimulated primary exocytosis DMF, whereas contents tertiary granules, secretory vesicles, only observed dbcAMP-differentiated cells. DMSO-differentiated exhibited highest capacity. Altogether our results reinforce fact depending on differentiating agent used, represent useful functions bypass difficulties inherent these