Model of chronic systolic and diastolic dysfunction after cryothermia-induced myocardial necrosis in rats.

摘要: BACKGROUND AND PURPOSE Left ventricular dysfunction following myocardial infarction is the most important predictor of adverse prognosis. Novel treatment options in require an appropriate experimental model with a standardized, hemodynamically relevant injury. We evaluated cryoinjury rodents that allows quantitative analysis systolic and diastolic dysfunction. METHODS Anesthetized, orally intubated, ventilated Lewis rats (n = 12) underwent sternotomy. Myocardial necrosis was induced by use standardized cryolesion to obtuse margin left ventricle, freezing for 3 minutes -160 degrees C. performance analyzed at day 120 after cryoinjury. Sham-operated animals 10) served as controls. RESULTS Cryoinjured behaved normally gained weight up 120. Average heart cryoinjured significantly exceeded pressure systolic, diastolic, mean aortic pressures were lower 4 months cryoinjury, whereas end-diastolic increased. had reduced blood flow, well impaired maximal dP/dt during occlusion occlusion-provoked peak pressure. Analysis rates isovolumic decrease revealed significant reduction negative animals. Finally, time constants decline prolonged CONCLUSION Standardized cryothermia induces lesion results highly reproducible impairment days cryothermia. The ideally suited test novel therapeutic strategies