Mycobacterium tuberculosis Infects Dendritic Cells with High Frequency and Impairs Their Function In Vivo

摘要: Mycobacterium tuberculosis (Mtb) is thought to reside in macrophages, although infected dendritic cells (DCs) have been observed. Thus, cellular associations made, global characterization of the harboring Mtb lacking. We performed temporal and quantitative following aerosol infection mice by using GFP-expressing bacteria flow cytometry. discovered that infects phagocytic diverse phenotypes, predominant cell populations change with time, myeloid DCs are major population lungs lymph nodes. also found lung-draining node transported there from a CCL19/21-dependent mechanism transport transient phenomenon despite chronic infection. In addition, we subsets most efficacious stimulating Mtb-specific, TCR-transgenic CD4(+) T lymphocytes not scarce or absent mice. Finally, lung at high frequency relatively ineffective Ag-specific lymphocytes, obtained evidence live can inhibit MHC class II Ag presentation without decrease surface expression II. These results indicate targets DC migration vivo promote persistent