Cell free circulating tumor nucleic acids, a revolution in personalized cancer medicine.

摘要: Innovative diagnostics are becoming an essential component in personalized cancer medicine. These increasingly based on cell-free nucleic acids and membrane vesicles. Isolating sequencing cell free circulating DNA (cfDNA) plasma may progressively substitute tumor biopsies. A small albeit now detectable fraction of cfDNA correspond to (ctDNA). In this review, we describe the pre-analytical procedures for collecting ctDNA from plasma, since these should be optimized within laboratories depending available infrastructures. We also provide overview technological breakthrough Isolation instance digital PCR methods next generation techniques discuss their key challenges. The clinical implementations liquid biopsy more specifically management reviewed. predict near future, will used routinely guide treatment a new approach personalize precision