Changes in the synthesis of actin and other cell proteins after stimulation of serum-arrested cells

摘要: Abstract The synthesis of both cytoplasmic and nuclear proteins has been studied as quiescent, serum-deprived Swiss mouse 3T3 cells are stimulated to transit the cell cycle. In serum-arrested a 200,000 dalton protein 51,000 were found be preferentially synthesized. serum-stimulated first major whose was seen increase had molecular mass 42,000 daltons. This also showed greatest change in during from G0 S phase. Its rose maximum 4--6 hr after stimulation then declined entered The present extracts. It identified actin on basis its mobility sodium dodecyl sulfate isoelectric focusing polyacrylamide gels. Other synthesized by masses 57,000 daltons (cytoplasmic), 33,000 (cytoplasmic nuclear), 15,000 (nuclear). increased gradually remained high began preferential these provides biochemical markers for transition quiescence proliferation.