Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media

作者: Hovsep Melkonyan , Samuil Umansky , Erik Meyer , William John Feaver

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摘要: This invention provides compositions and methods for rapid separation, isolation purification of nucleopolymers, especially nucleic acids, from biological samples. using anionic exchange media. The method the present can utilize commercially available strong or weak anion exchanger materials with selected solutions known ionic strength adsorption elution. medium/nucleoprotein bound complex may be optionally stored transported, but is subsequently treated appropriate eluents to remove any undesirable proteins, including destabilizing enzymes, inorganic salts like. partially purified also transported prior further processing. instant particularly advantageous as it means soluble acids obtained samples fluids comparatively large volume, allowing ease handling storage, stabilizing against degradation analysis. It permits identification shorter fragments bodily which, up till now, had not been identified. widely useful a prepare formats that are convenient in diagnostic analytical methods, those rely on detection, characterization acid sequences, body fluids.

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