Structural compartments within neurons: Developmentally regulated organization of microfilament isoform mRNA and protein

摘要: Abstract The microfilament system is thought to be a crucial cytoskeletal component regulating development and mature function of neurons. intracellular distribution the isoform components, actin tropomyosin (Tm), in neurons primarily vivo, has been investigated at both mRNA protein level using specific riboprobes antibodies. Our vivo vitro studies have identified least six neuronal compartments based on localization: developing soma, growth cone, axon hillock/proximal axon, somatodendritic axonal pole soma. Protein localization patterns revealed that isoforms were frequently distributed over wider area than their respective mRNAs, suggesting targeting may influence, but do not absolutely determine, location. Tm4 Tm5 showed identical neuron distinct patterns. We suggest this instance location best viewed as regulated site synthesis assembly, rather regulator per se. In addition, β-actin locations developmentally regulated, possibility environmental signals modulate mRNAs proteins. Thus, positional translation act concert with transport regulate composition consequently structure.