Long-Term and Programmable Bacterial Subculture in Completely Automated Microchemostats

摘要: A controllable microchemostat can provide an ideal, powerful means to study the growth behavior of microorganisms by improving conventional macroscale chemostat. However, a challenge remains for implementing both continuous and active population control at same time because they keep communicating with nearby culture environments regulating their metabolism. Here, we present novel that enables reversible bacterial isolation, chemical refreshment, dynamic physicochemical stimulation. The not only controls subculture conditions in completely automated programmed manner but it also makes possible manipulate populations from single bacterium ultrahigh density long-term periods ultralow reagent consumption. Moreover, situ measurement feedback through various programming modes ...