Hepatocyte Growth Factor Region Specifically Stimulates Gastrointestinal Epithelial Growth in Primary Culture

摘要: Abstract Hepatocyte growth factor (HGF) stimulated the of fetal rat gastrointestinal epithelial cells in primary culture with a clear dose-response relationship The response to mitogenic activity HGF was different among region gastro-intestinal tract; glandular stomach most responsive, followed by intestine and forestomach. interaction other factors inducing also depending on type cells, indicating region-specific regulation tract. Analyses using Northern blot RT-PCR revealed that mRNA expressed only mesenchymes but not epithelia tract while expression c-met (HGF receptor) gene observed both tissues. These results suggest secrete which stimulates attaching paracrine mechanism is controlled regulatory mechanism.