Imaging neuronal development with magnetic resonance imaging (NMR) microscopy.

摘要: An ideal technique for following the development of vertebrate nervous system would allow cells to be followed at resolution light microscopy depths several millimeters into tissue. This permit critical events cellular or sub-cellular even deep within developing organism. To date, no has emerged with all needed properties. Light can follow a cell and its descendants after they have been labeled by either infection embryonic recombinant retrovirus microinjection individual precursor enzymes fluorescent dyes. However, cannot image deeper than few hundred micrometers an embryo due scattering aberrations in objective lenses other optics. Magnetic resonance imaging (MRI) does not suffer from these limitations, routinely being used 3 dimensions through specimens as large adult humans. it is relatively slow and, implemented achieve resolution. Here, we present our attempts meet technical challenges posed vivo MRI microscopy. As example both progress future challenges, images frog over day time course.