Tuning lipid mixtures to induce or suppress domain formation across leaflets of unsupported asymmetric bilayers

摘要: Plasma membranes of cells are asymmetric in both lipid and protein composition. The mechanism by which proteins on sides the membrane colocalize during signaling events is unknown but may be due to induction inner leaflet domains outer leaflet. Here we show that liquid form Montal-Mueller planar bilayers one leaflet's composition would phase-separate a symmetric bilayer other's not. Equally important, tuning second leaflet, able suppress first When present membranes, each contains regions three distinct compositions, implying strong interleaflet interactions. Our results mechanisms domain between leaflets cell plasma do not necessarily require participation proteins. Based these findings, suggest could actively regulate function modulating local or