Proteomics on Brefeldin A-Treated Arabidopsis Roots Reveals Profilin 2 as a New Protein Involved in the Cross-Talk between Vesicular Trafficking and the Actin Cytoskeleton

摘要: The growing importance of vesicular trafficking and cytoskeleton dynamic reorganization during plant development requires the exploitation novel experimental approaches. Several genetic cell biological studies have used diverse pharmaceutical drugs that inhibit secretion to study these phenomena. Here, proteomic biology approaches were applied effects brefeldin A (BFA), an inhibitor vesicle recycling secretion, in Arabidopsis roots. main aim this was obtain overview proteins affected by BFA, but especially identify new involved its cross-talk actin cytoskeleton. results showed BFA altered caused formation BFA-compartments which accompanied differential expression several root cells. Some BFA-up-regulated belong class proteins, such as V-ATPase reversibly glycosylated polypeptide, while others, profilin 2 elongation factor 1 alpha, are rather remodeling Upregulation verified immunoblot live imaging at subcellular level. latter approach also revealed accumulated BFA-treated Thus, seems be between cytoskeleton, a BFA-dependent manner.