Automated parallel DNA sequencing on multiple channel microchips.

摘要: We report automated DNA sequencing in 16-channel microchips. A microchip prefilled with sieving matrix is aligned on a heating plate affixed to movable platform. Samples are loaded into sample reservoirs by using an eight-tip pipetting device, and the chip docked array of electrodes focal plane four-color scanning detection system. Under computer control, high voltage applied appropriate programmed sequence that injects separates samples. An integrated confocal fluorescent detector automatically scans all 16 channels. The system routinely yields more than 450 bases 15 min In best case base-calling program, 543 have been called at accuracy >99%. Separations, including loading injection, normally completed less 18 min. advantages capillary electrophoresis chips include uniform signal intensity tolerance template concentration. To understand fundamentals these unique features we developed theoretical treatment cross-channel injection call differential concentration effect. present experimental evidence consistent predictions theory.