Direct cloning of the trxB gene that encodes thioredoxin reductase
作者： M Russel , P Model
DOI: 10.1128/JB.163.1.238-242.1985
关键词:
摘要: A strain was constructed which contains mutations in the genes encoding thioredoxin (trxA) and reductase (trxB) such that filamentous phage f1 cannot grow. The complementation of either mutation with its wild-type allele permits growth. We used this to select contain a cloned trxB gene. location gene on fragment determined, protein product identified. Plasmid subclones overproduce reductase.
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