Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation.

摘要: Background: Radiation therapy (RT) is a well established therapeutic modality for the treatment of solid tumors. In particular, post-operative RT considered standard adjuvant to surgery since its ability prolong median survival patients with malignant astrocytoma has been shown; nevertheless ionizing radiation (IR) fails in considerable number patients. Materials and Methods: Using an ADF human cell line molecular mechanisms involved DNA damage induced by fractionated irradiation (FIR) single IR have investigated. Results: FIR inhibited growth line. FACS analysis revealed that treatment, but not inhibition associated induction apoptosis. Apoptosis was related caspase-3 activation reactive oxygen species (ROS) generation. ROS formation depends on up-regulation cytochrome P450 enzyme gene. On contrary, 12.5 Gy necrotic death up-regulating HSPD1, HSPCB, HSPCA HSPB1 genes. Conclusion: through ROS-mediated