Isolation and culture of the islets of Langerhans of the guinea pig

作者: Stanislaw Moskalewski

DOI: 10.1016/0016-6480(65)90059-6

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摘要: Abstract Islets of Langerhans guinea pigs were isolated by means collagenase and identified with a dissecting mieroscope. Organ culture the islets was started in natural medium various glucose concentrations. The examined histologically their general morphology noted, as well state specific granules alpha beta cells. Both types cell underwent partial degranulation. At high concentration cells more degranulated nuclei noticeably hypertrophied. No distinct differences at low concentrations could be detected. It is concluded that influence upon islands vivo vitro prineiple similar.

参考文章(32)
van Limborgh, van Fassen, The asymmetry of the gonads in duck embryos experimentally turned on their right side. Acta Morphologica Neerlando-Scandinavica. ,vol. 3, pp. 79- 91 ,(1960)
M. Laskowski, M. Laskowski, Naturally occurring trypsin inhibitors. Advances in Protein Chemistry. ,vol. 9, pp. 203- 242 ,(1954) , 10.1016/S0065-3233(08)60207-7
M. L. Cameron, J. E. Steele, Simplified aldehyde-fuchsin staining of neurosecretory cells. Biotechnic & Histochemistry. ,vol. 34, pp. 265- 266 ,(1959) , 10.3109/10520295909114686
BERNT HÖKFELT, GÖSTA HULTQUIST, The Influence of Hypoglycemia on the Histolog‐ic Structure of the Alpha Cells in the Pancreas. Acta Physiologica Scandinavica. ,vol. 43, pp. 8- 14 ,(1958) , 10.1111/J.1748-1716.1958.TB01573.X
R. E. Haist, ISLET CELL FUNCTION Annals of the New York Academy of Sciences. ,vol. 82, pp. 266- 286 ,(2006) , 10.1111/J.1749-6632.1959.TB44908.X
O TROWELL, The culture of mature organs in a synthetic medium Experimental Cell Research. ,vol. 16, pp. 118- 147 ,(1959) , 10.1016/0014-4827(59)90201-0
Birger Petersson, Claes Hellerstr�m, Bo Hellman, Some characteristics of the two types of A-cells in the islets of Langerhans of guinea-pigs. Cell and Tissue Research. ,vol. 57, pp. 559- 566 ,(1962) , 10.1007/BF00339882
Birger Petersson, Bo Hellman, EFFECTS OF LONG TERM ADMINISTRATION OF GLUCAGON ON THE PANCREATIC ISLET TISSUE OF RATS AND GUINEA-PIGS. European Journal of Endocrinology. ,vol. 44, pp. 139- 149 ,(1963) , 10.1530/ACTA.0.0440139
R. R. Bensley, Studies on the pancreas of the guinea pig American Journal of Anatomy. ,vol. 12, pp. 297- 388 ,(1911) , 10.1002/AJA.1000120304