Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of xylose and glucose into ethanol and other products

摘要: Lactobacillus buchneri strain NRRL B-30929 was isolated from a fuel ethanol production facility. This heterofermentative, facultative anaerobe can utilize xylose as sole carbon source and tolerates up to 12% ethanol. Carbohydrate utilization (API, Biomerieux) Phenotype Microarrays™ (PM, Biolog) analyses indicated that the is able metabolize broad spectrum of sources including various monosaccharides (C5 C6), disaccharides oligosaccharides, with better rates under anaerobic conditions. In pH-controlled bioreactors, bacterium consumed glucose simultaneously at high concentrations (125 g L−1, pH 6.0). The major fermentation products were lactate (52 L−1), acetate (26 L−1) (12 L−1). ferments alone (pH 4.0) into molar ratio 1.03:1. will be further explored via genetic engineering for potential applications in biomass conversion.