Unique odd-chain polyenoic phospholipid fatty acids present in chytrid fungi.

作者: Philips O. Akinwole , Emilie Lefevre , Martha J. Powell , Robert H. Findlay

DOI: 10.1007/S11745-014-3934-3

关键词:

摘要: Chytrid fungi are ubiquitous components of aquatic and terrestrial ecosystems yet they remain understudied. To investigate the use phospholipid fatty acids as phenotypic characteristics in taxonomic studies biomarkers for ecological studies, 18 chytrid isolated from soil to freshwater samples were grown defined media their acid profile determined. Gas chromatographic/mass spectral analysis indicated presence typically associated with fungi, such 16:1(n-7), 16:0, 18:2(n-6), 18:3(n-3) 18:1(n-9), 18:0, well as, a number odd-chain length acids, including two polyunsaturated C-17 acids. Conversion 3-pyridylcarbinol ester facilitated GC–MS determination double-bond positions these identified 6,9-17:2 [17:2(n-8)] 6,9,12-17:3 [17:3(n-5)]. best our knowledge, this is first report phospholipids fungi. Cluster PLFA profiles showed sufficient correlation phylogeny warrant inclusion lipid species descriptions several restricted phylogenetic distributions suggests usefulness studies.

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