Klf5maintains the balance of primitive endoderm versus epiblast specification during mouse embryonic development by suppression ofFgf4
作者： Takuya Azami , Tsuyoshi Waku , Ken Matsumoto , Hyojung Jeon , Masafumi Muratani
DOI: 10.1242/DEV.150755
关键词:
摘要: The inner cell mass of the mouse blastocyst gives rise to pluripotent epiblast (EPI), which forms embryo proper, and primitive endoderm (PrE), extra-embryonic yolk sac tissues. All cells coexpress lineage markers such as Nanog Gata6 at embryonic day (E) 3.25, EPI PrE precursor eventually segregate exclusively express Gata6, respectively. Fibroblast growth factor (FGF)-extracellular signal-regulated kinase (ERK) signalling is involved in segregation lineages; however, mechanism Fgf4 regulation poorly understood. Here, we identified Klf5 an upstream repressor Fgf4Fgf4 was markedly upregulated knockout (KO) embryos E3.0, downregulated overexpressing Furthermore, KO blastocysts showed skewed specification phenotypes, similar FGF4-treated preimplantation embryos, Inhibitors FGF receptor (Fgfr) ERK pathways reversed blastocysts. These data demonstrate that suppresses Fgf4-Fgfr-ERK signalling, thus preventing precocious activation programme.
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