De novo assembly of wheat root transcriptomes and transcriptional signature of longitudinal differentiation.
作者： Ghana Shyam Challa , Wanlong Li
DOI: 10.1371/JOURNAL.PONE.0205582
关键词:
摘要: Hidden underground, root systems constitute an important part of the plant for its development, nourishment and sensing soil environment around it, but we know very little about genetic regulation in crop plants like wheat. In present study, de novo assembled transcriptomes reference cultivar Chinese Spring from RNA-seq reads generated by 454-GS-FLX HiSeq platforms. The FLX were into 24,986 transcripts with completeness 54.84%, 91,543 high-confidence protein-coding transcripts, 2,404 low-confidence 13,181 non-coding >90%. Combining assemblies, a transcriptome 92,335 ORF-containing transcripts. Approximately 7% coding ~2% are not current wheat genome assembly. Functional annotation both assemblies showed similar gene ontology patterns that ~7% >5% root-specific. Transcription quantification identified 1,728 differentially expressed between tips maturation zone, functional these captured transcriptional signature longitudinal development root. With transcriptomic resources developed, this study provided first view under different developmental zones laid foundation molecular studies growth using reverse approach.
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