Calcium phosphate nanoparticles: a study of their synthesis, characterization and mode of interaction with salmon testis DNA

摘要: Calcium phosphate nanoparticles (CPNPs) are presently emerging as a second generation vector for efficient delivery and stabilization of nucleic acids inside cells, although the detailed mode interaction between CPNPs DNA is still obscure. This study discloses some features interaction. For this study, we synthesized by modified co-precipitation method characterized particles different techniques such dynamic light scattering, X-ray diffraction, electron dispersive spectroscopy, Fourier transform infra-red differential thermal thermo-gravimetric analysis, atomic force, scanning transmission microscopy. The characterization studies showed that were spherical in shape, about 45 nm size composed hydroxyapatite form calcium phosphate; almost 90% starting materials converted to (NPs). aspects salmon testis investigated using UV-Vis spectrophotometry, circular dichroism, spectrometry, denaturation, microviscometry, agarose gel electrophoresis, cyclic voltammetry force results revealed interacted with ∼1 : 3.3 stoichiometry binding constant order 104 M−1 through groove-interacting single nanoparticle covered 6.2 base pairs chain. Moreover, was spontaneous, cooperative, exothermic enthalpy-driven electrostatic nature also evident; however, non-polyelectrolyte contribution dominant. finally caused an increase melting temperature from 70.8 °C 75 alteration its secondary structure naturally occurring B-form C-form.