The DNA-binding protein of Pf1 filamentous bacteriophage: amino-acid sequence and structure of the gene.

摘要: The amino-acid sequence of the single-stranded DNA-binding protein bacteriophage Pf1 and nucleotide corresponding gene have been determined. has 144 amino acids a molecular weight 15 400; consists 435 nucleotides. was determined by Edman degradation, carboxypeptidase A, B, P digestion intact peptides derived chymotrypsin, Staphylococcus aureus V8 protease, trypsin digestion. dideoxy method after random cloning fragments DNA into M13. No homology could be established between Pseudomonas aeruginosa-specific fd Escherichia coli.