DNA from dead cancer cells induces TLR9-mediated invasion and inflammation in living cancer cells

摘要: TLR9 is a cellular DNA-receptor, which widely expressed in breast and other cancers. Although synthetic TLR9-ligands induce cancer cell invasion vitro, the role of pathophysiology has remained unclear. We show here that living cells uptake DNA from chemotherapy-killed cells. discovered such induces TLR9- cathepsin-mediated To study whether this phenomenon contributes to treatment responses, triple-negative, human MDA-MB-231 stably expressing control, or siRNA were inoculated orthotopically into nude mice. The mice treated with vehicle doxorubicin. tumor groups exhibited equal decreases size response However, while weights vehicle-treated similar, bearing control tumors became significantly more cachectic doxorubicin, as compared similarly tumors, suggesting TLR9-mediated inflammation at site tumor. In conclusion, our findings propose released significant influence on biological effects Through these mechanisms, expression may affect responses chemotherapy.