Further evidence of thermostability variation within electrophoretic mobility classes of enzymes.

摘要: Two Drosophila melanogaster strains, each heterozygous for “fast” and “slow” alleles at the Adh locus, having balanced second chromosomes, were found to differ in apparent thermostability of slow allozyme. The two strains crossed, F1heterozygotes separated on basis origin allele. After electrophoresis, cellulose acetate strips treated 1 1/2 min 35 C. putatively more sensitive allozyme showed a strikingly greater response heat. These findings further support conclusion that electrophoretically cryptic allelic differences exist which are expressed differences. Further application this approach has revealed one similar three cases relatively resistant fast ADH wild-caught flies.