Hexose phosphorylation and the putative calcium channel component Mid1p are required for the hexose-induced transient elevation of cytosolic calcium response in Saccharomyces cerevisiae.

摘要: Summary Saccharomyces cerevisiae responds to environ- mental stimuli such as an exposure pheromone or hexoses after carbon source limitation with a tran- sient elevation of cytosolic calcium (TECC) response. In this study, we examined whether hexose transport and phosphorylation are necessary for the TECC We found that mutant strain lacking most known transporters was unable carry out response when exposed glucose. A lacked ability phosphorylate glucose respond addition, but displayed normal addi- tion galactose. These results indicate uptake required trigger hexose-induced also significantly smaller than level environmental reduced, abolished in mid1 subunit high-affinity channel yeast plasma membrane. all is mediated by influx from extracellu- lar space. Our transient increase membrane permeability may be linked accumulation Glc-1-P (or related metabolite) yeast.