Transcription of the dystrophin gene in human muscle and non-muscle tissues

摘要: The gene that is defective in patients with Duchenne and Becker muscular dystrophy consists of about 60 short exons scattered along a gigantic DNA region spans some 2 megabase pairs1,2. encoded protein, dystrophin, was recently characterized as component muscle intracellular membranes low abundance3,4. dystrophin messenger RNA difficult to study both normal pathological tissue specimens because it large (14 kilobases) scarce (0.01–0.001% total mRNA)2. We report here efficient vitro co-amplifications the mRNAs reporter gene, aldolase A, by poly-merase chain reaction procedure5 enables us obtain quantitative estimate transcript. A processed, transcribed segment thus detected 13 different human tissues. It ranged from 0.02–0.12% mRNA skeletal 25,000 times less lymphoblastoid cells.