Nestin-Expressing Stem Cells from the Hair Follicle Can Differentiate Into Motor Neurons and Reduce Muscle Atrophy after Transplantation to Injured Nerves
作者： Fang Liu , Chuansen Zhang , Robert M. Hoffman
DOI: 10.1089/TEN.TEA.2012.0657
关键词:
摘要: We have previously shown that nestin-expressing hair follicle stem cells from the mouse and human are multipotent can differentiate into many cell types, including neurons glial cells. The effect nerve spinal cord repair upon transplantation in models. In present study, expressing red fluorescent protein (RFP) were induced by retinoic acid fetal bovine serum to then transplanted together with Matrigel transected distal sciatic or tibial stump of transgenic nude mice ubiquitously green (GFP). Control only. appeared neuron like, large round nuclei long extensions. Immunofluorescence staining showed some expressed marker Tuj1 as well motor markers Isl 1/2 EN1. These contacted each other host fibers. Two weeks post-transplantation, fibers had greater expression neurotrophic factor-3 than those Matrigel-only mice. Muscle fiber areas plus Matrigel-transplanted animals much bigger after 4 weeks. results suggest reduce muscle atrophy transection. This study demonstrates a new accessible source injury.
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