The extracellular nuclease activity of Micrococcus sodonensis. IV. Physical studies, characterization as a glycoprotein and involvement with the cell wall.

摘要: 1. 1. The molecular weight and s20,w of Micrococcus sodonensis nuclease were found to be concentration dependent extrapolation zero yielded values 500 000 2.2 S, respectively. dependence behaviour the peak in sedimentation velocity experiments are indicative an asymmetric molecule. 2. 2. enzyme was shown a glycoprotein consisting 21% carbohydrate covalently linked protein moiety. Glucosamine, glucose, galactose rhamnose identified as component sugars present ratio 4:2:1:1. A serine-glucosamine linkage involving at least 80% serine residues demonstrated. 3. 3. Amino acid analyses showed low cysteic residue contain relatively high amounts serine, threonine, glycine proline. Unexpected for tryptophan result presence amino sugar. 4. 4. moiety purified related both chemically immunologically cell wall active bound stable configuration wall. 5. 5. possible role formation is discussed.