De novo transcriptome analysis of Rhizophora mucronata Lam. furnishes evidence for the existence of glyoxalase system correlated to glutathione metabolic enzymes and glutathione regulated transporter in salt tolerant mangroves.

摘要: Abstract The accumulation of a metabolic by product – methylglyoxal above minimal range can be highly toxic in all organisms. Stress induced elevation inactivates proteins and nucleic acids. Glutathione dependent glyoxalase enzymes like I II together with glutathione independent III play inevitable role detoxification. Glyoxalase genes are generally conserved but obvious exceptions. Mangroves being potent harsh land inhabitants, their internal organelles constantly been exposed to elevated levels methylglyoxal. First foremost it is important detect the presence glyoxalases mangroves. De novo transcriptome analysis mangrove species Rhizophora mucronata Lam., identified eleven putative (RmGLYI-1 5, RmGLYII-1 5 RmGLYIII). Molecular characterization proposed PLN02300 or PLN02367 as key domains RmGLYI proteins. They possess molecular weight ranging from 26.45 32.53 kDa may localize cytosol chloroplast. RmGLYII 28.64–36 kDa, carrying PLN02398 PLN02469 expected localized diverse cellular compartments. Cytosolic RmGLYIII DJ-1/PfpI domain carries 26.4 kDa. Detailed structural revealed monomeric nature RmGLYI-1 whereas found homodimer. phylogenetic multiple sequence alignment specified metal ion/substrate binding residues RmGLY Estimation relative expression under salt stress indicated prominence over RmGLYIII. aforementioned supported difference regulated transporter protein.