In vitro dimerization of HIV-2 leader RNA in the absence of PuGGAPuA motifs.

摘要: Retroviral particles contain a dimeric genome consisting of two full-length, noncovalently linked RNA molecules. Linkage the genomes is thought to be critical for productive reverse transcription reaction and may increase genetic recombination rates. The molecular nature dimer linkage structure (DLS) poorly understood. It was recently shown that in vitro synthesized retroviral transcripts can dimerize absence protein factors. We studied dimerization human immunodeficiency virus type 2 (HIV-2) RNA. Specific HIV-2 RNAs observed upon incubation at 37 degrees C high-salt buffer. Previously, physical biochemical studies have mapped structures leader close gag open reading frame. In this study, we found efficient containing only 5' terminal 255 nucleotides Therefore, it seems likely multiple signals are present Th...