Fluorometry of citrate in serum, with use of citrate (pro-3S)-lyase.

摘要: We describe a procedure for enzymatic assay of citrate in human serum. The is degraded to acetate and oxaloacetate with oxaloacetate-lyase ( pro -3 S -CH2 · COO- → acetate) (EC 4.1.3.6). Some loses CO2 form pyruvate. Addition malate lactate dehydrogenases 1.1.1.37 1.1.1.27) permits determination the pyruvate generated, thus concentration. decrease NADH concentration measured fluorometrically. Results obtained 30 consecutive sera by this were compared which converted pentabromoacetone. There was no statistically significant difference values two procedures. range (mean ± 2 SD) found from 25 blood donors 12.8-27.2 mg/liter (mean, 19.0 mg/ liter). Serum as both procedures during glucose tolerance test decreased initial under influence administered (and endogenous insulin). Insulin concentrations also these glucose-tolerance tests. Citrate remain subnormal after insulin return their values. This accords published reports.